PIG3 (Colorimetric)

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PIG3 (p53-Inducible Gene 3) ELISA Assay Kit (Colorimetric)

PIG3 Assay is a non-isotopic, non-radioactive immunoassay for the quantitation of the p53 inducible gene 3 (PIG-3) - a long-lived reporter of transient p53 activation.¹ The assay is suitable for human sera, plasma, tissue culture supernatants and cellular extracts or lysates.

Features: - High sensitivity - 4 deg C storage with long shelf life - Non-radioactive - 2.5 hour protocol - Suitable for sera, plasma, culture supernatants, cellular extracts/lysates - HTS compatible format	Performance Summary: - Sensitivity: 40 pg/ml - Recovery: serum, >98%; plasma, >98% Precision Studies - Intra-assay C.V.'s: <3% mid-level - Inter-assay C.V.'s: <4% mid-level
	*Ordering Information:*
	Cat. No. X1326K1 X1326k2 x1326k3	Size 1 x 96 wells 2 x 96 wells 10 x 96 wells
	For larger quantity pricing, please contact Customer Service

Legend:
PIG-3 assay. Graphs show pooled coefficients of variation and between assay coefficients of variation (top), senitivity of assay with ng/ml of PIG-3 versus absorbance at 450-550 nm (middle) and standard curve with ng/ml of PIG-3 versus absorbance at 450-550 nm (bottom).

Protocol Summary: Not to be used in place of detailed protocol:

1. Equilibrate kit to room temperature
2. Add samples and standards to wells Add detector antibody to wells	• Incubate 2 hours at room temperature
3. Wash 3 times
4. Add conjugate to all wells	• Incubate 30 minutes at room temperature
5. Wash 3 times
6. Add substrate to all wells	• Incubate 30 minutes at room temperature
7. Add stop solution to all wells
8. Read plate(s) at 450 nm/550 nm