Apo Logix sulforhodamine Caspase Detection Kit

Myeloperoxidase Detection

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APO LOGIX Sulforhodamine Caspase Detection Kit ™
Apoptosis detection and in situ labeling of active caspase in live cells.

Key Benefits:

Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition.
Cell permeablity permits direct visualization of cytosolic apoptotic events.
Apoptotic cell population does not diminish over time.
Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure.
Works in diverse cell lines: human, rodent, Drosophila.
Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells.
Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments.
Applications - Works with fluorescence microscope, 96-well fluorescence plate readers
Yields both quantitative and qualitative results. Gives strong signal with little background noise.

Assay Principle

APO LOGIX SR kits contain a generic sulforhodamine labeled caspase inhibitor (sulforhodamine-peptide-fluoromethyl ketone). This reagent is cell permeable and is used on whole cells to detect apoptosis. Apoptotic cells are detected by a fluorescence plate reader or fluorescence microscope using an excitation source at 550nm and measuring emission at 595nm. The assay takes about 1 hr to complete

Kit contents

Lyophilized SR-VAD-FMK
10X Wash Buffer
10X fixative

The following kits are available:
Product	Catalog No.	Size (No. of Tests)	Price (EUR €)
SR-VAD-FMK Poly caspase detection kit	SR100-1	25	€195
SR-VAD-FMK Poly caspase detection kit	SR100-2	100	€525
SR-DEVD-FMK Caspase 3 detection kit	SR200-1	25	€195
SR-DEVD-FMK Caspase 3 detection kit	SR200-2	100	€525

Jurkat cells stimulated with staurosporine for 2 hours and then labeled with SR-VAD-FMK.

Left side: 30X phase contrast

Right side: 30X fluorescence microscope. Excitation: 550nm emission > 580nm.

Jurkat cells stimulated with staurosporine for 2 hours. Cells were then stained with SR-VAD-FMK for 1 hour and read in a 96 well fluorescence plate reader.