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  Tel + 32 16 58 90 45 

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Hydroxyl / Peroxynitrite Detection Kit
Fluorescent Hydroxyl (•OH) / Peroxynitrite (ONOO -) Detection Kit
Key Benefits:
  • Quenched Cell permeable dye
  • Can be used with Cell Lysates, Tissue Homogenates
  • One Step, No wash Homogenous assay.
  • Adaptable to High throughput assay platforms
  • Can monitor multiple time points to follow real time kinetics
  • Non-destructive cell based assay allows monitoring of additional parameters
  • Applications - Fluorescence plate reader / Flow cytometer / Fluorescence Microscope
Introduction:
A new novel probe, Hydroxyphenyl fluorescein (HPF), developed by Tetsuo Nagano et. al. (1), is a highly selective probe for the detection of highly Reactive Oxygen Species (hROS). It is a cell permeable highly sensitive fluorescent probe for hydroxyl radical (OH•), and peroxynitrite (ONOO-) detection. It has little reactivity towards other hROS such as: hypochlorite (-OCl), singlet oxygen (021), superoxide (02-•), hydrogen peroxide (H202), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1.
Assay Principle:
Reactivity:
Reactivity Profile of HPF:
ROS (RFU)
 		 HPF (RFU)
Ex:499 Em:515
 		 DCFH-DA (RFU)
Ex:500 Em:520
 
Hydroxyl Radical: •OH
 		 730 7400
Peroxynitrite: ONOO- 120
 		 6600
Hypochlorite: -OCl 6 86
Oxygen Radical: 1O2 5 26
Superoxide: O2-• 8 67
Hydrogen Peroxide : H202 2 190
Nitric Oxide: NO 6 150
Alkylperoxyl Radical: ROO• 17 710
Autoxidation 1 2000
References:
  1. Ken-ichi Setsukinai, Yasuteru Urano, Katsuko Kakinuma, Hideyuki J. Majima , and Tetsuo Nagano. Development of Novel Fluorescence Probes That Can Reliably Detect Reactive Oxygen Species and Distinguish Specific Species. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 278, No. 5, Issue of January 31, pp. 3170–3175, 2003

    Manufactured: by Daiichi Pure Chemicals Co. Ltd. Japan

 

The following kits are available:
Catalog #
 		 Contents Size* Price
FLHPF 100-2
 		 HPF 1 vial 150 Tests * €195
Not for Sale in Japan

*Test size will depend on dilution and volume used per test. For example:
Dilution of HPF
 		 Volume of test Numer of tests
1:500 (10mM final)
 		 0.2 mL 147
1: 1000 (5mM final) 0.2mL 294