Nicotinamide adenine dinucleotide
phosphate (NADP+) is used in anabolic reactions, such as
lipid and nucleic acid synthesis, which require NADPH as a
reducing agent. NADPH is the reduced form of NADP+, and NADP+
is the oxidized form of NADPH. In cells, NADPH plays the
role of a carrier of reducing power and is primarily
involved in maintaining optimal redox metabolism. A
simplified assay for the measurement of NAD and NADP is
critical to understanding the roles of these pyridine
nucleotides in normal and abnormal cells.
NADPH is produced in the oxidative phase of the pentose
phosphate pathway in cells, a multifunctional pathway whose
primary purpose is to generate reducing power in the form of
NADPH. NADPH is a cofactor for enzymes that synthesize
energy-rich molecules and provide the reducing equivalents
for the oxidation-reduction involved in protecting the cell
from the toxicity of reactive oxygen species (ROS) and NADPH
oxidase-dependent ROS generation. Both NAD and NADP have
been shown to influence hemoglobin affinity for oxygen in
erythrocytes. In plant cells, NADPH is used as the reducing
power for the biosynthetic reactions in the Calvin cycle of
photosynthesis (1-2).
Cell Technology’s Fluoro NADP/NADPH provides a highly
reliable, sensitive fluorometric assay for the
quantification of NADP, NADPH and their ratio in biological
samples.
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