Cellufine Mini-Column

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Cellufine Mini-Column
Effective tool for protein purification.

Cellufine Mini-Columns offer convenience and expedience for isolating, purifying, and concentrating biomolecules from aqueous samples.

Application Data:

Dynamic Binding Capacity (DBC) of lysozyme on Cellufine Sulfate

No significant change in DBC was observed over a flow range of 0.5 to 5 ml/min

NEW Influenza Virus Separation by Cellufine Sulfate Mini-Column!

Conditions:

Volume of Cellufine Sulfate:1ml （ID 8×20 mm)
Liner Velocity: 120cm/hr
Equilibration buffer: 0.01M Phosphate buffer, pH 7.4
Washing buffer: 0.01M Phosphate buffer, 0.19M NaCl, pH7.2
Elution buffer: 0.01M Phosphate buffer, 3M NaCl, pH7.0
Virus Strain: A/duck/Hokkaido/Vac-1/04 (H5N1) β-Propiolactone
--- Allantoic Fluid was filtered after centrifuged
(Centrifugation: 8,000g×30min, Filter:0.45μm Cellulose Acetate)
Sample Volume: 6 ml : (The virus sample from professor Kida.(Hokkaido Univ. Graduate school of veterinary medicine. )

How to Use: