Myeloperoxidase Detection 

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Block Buffers
Improves Assays:
  • Increases the Sensitivity of the Assay. This is done by stabilizing the antigenic and functional regions of the absorbed protein.
  • Reduces Background Noise. Block buffers inhibit nonspecific binding to uncoated regions of the plate and non-specific binding sections of the absorbed proteins. This leads to lower background noise and high specific signal.
  • Increases Efficiency and Extends the Shelf-Life of the Coated Plates. With a longer shelf-life, plates may be prepared in large batches to be used over time. Large batch sizes will increase efficiency and reduce plate-to-plate variability to increase consistency.
  • Conserves Valuable Reagents. By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when the proper blocker is used.
Blockers Available:
EBB100 General-Purpose Blocker with BSA-Based Blockers
EBB200 Non-Mammalian-Based Blocker
EBB300 Synthetic Blocker with Tween
Directions:
Once your plate has been coated with an antibody or antigen, aspirate or dump out the coating solution. Then, simply pipette 300-400uL per well of block buffer and let it incubate (Cell Technology recommends a minimum incubation of 3 hours).
EBB100 General-Purpose Blocker with BSA-Based Blockers
General-Purpose Blocker contains a mammalian protein blocking agent (based on BSA) suitable for most monoclonal and polyclonal antibody capture ELISA formats, as well as peptide and protein antigen-down ELISA formats. This unique block buffer contains proprietary protein stabilizers that provide a long-term stabilizing environment for dried antigen or antibody coat proteins, while minimizing nonspecific binding interactions during the assay process.
Catalog Number Size Plates at 300uL/well
(28.8mL/plate)
 		 Plates at 400uL/well
(38.4mL/plate)		 Price
EBB100-1 100 3 2 €45.00
EBB100-2 500
 		 16 12 €120.00
EBB100-3 1,000 33 25 €199.00
EBB100-4 10,000 330 250 €1900.00
EBB200 Nonmammalian-Based Blocker
Cell Technology's Nonmammalian-Based Blocker contains a proprietary non-mammalian formulation based on fish proteins, plus molecular stabilizers that provide a high degree of blocking efficiency. EBB200 was designed for antigen-down ELISA formats, and for sandwich assays with high background problems. This formulation is a heterogeneous mixture of small molecules capable of blocking both the nonspecific binding sites of the absorbed protein, as well as the unoccupied regions of the polystyrene plate that are not sterically accessible to larger traditional mammalian blocking reagents, thereby reducing background noise. The small size of these unique blockers results in minimal steric hindrance to key epitope regions of target coated proteins, thus enhancing specific signal generation.

EBB200 reduces the possibility of false-positives generated from endogenous antibodies in the sample reacting with plate blocking proteins. It is particularly useful when working with human, swine, and bovine serum samples, as there is little interaction with the blocking molecules resulting in lower backgrounds.

Catalog Number Size Plates at 300uL/well
(28.8mL/plate)
 		 Plates at 400uL/well
(38.4mL/plate)		 Price
EBB200-1 100 3 2 €45.00
EBB200-2 500
 		 16 12 €140.00
EBB200-3 1,000 33 25 €270.00
EBB200-4 10,000 330 250 €2500.00
EBB300 Synthetic Blocker
Cell Technology's Synthetic Blocker is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. Because Synthetic Blocker contains inert non-reactive blockers, it reduces the amount of nonspecific binding of enzyme-labeled conjugates to blocked plate surfaces. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives.
Catalog Number Size Plates at 300uL/well
(28.8mL/plate)
 		 Plates at 400uL/well
(38.4mL/plate)		 Price
EBB300-1 100 3 2 €45.00
EBB300-2 500
 		 16 12 €140.00
EBB300-3 1,000 33 25 €270.00
EBB300-4 10,000 330 250 €2300.00