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0032 (0)16 41 44 07 |
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APO ACTIVE 3 ™
Antibody Specific Active Caspase 3 Detection Kit
|
| Key Benefits: |
-
Highly specific for active human and murine caspase 3.
Other assays require the utilization of peptide based (DEVD)
reagents that tend to cross-react with caspase 7 and
other caspases.
-
Applications - Works with a fluoresence
microscope, 96 well plate reader, or flow cytometer.
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Yields both quantitative and qualitative results. Gives
a strong positive signal.
-
Can be used in conjunction with other antibodies or
stains.
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No need to make cell lysates or run western blots. Cells
can be fixed and analyzed later.
|
| Assay Principle |
|
Cell Technology ’s APO-ACTIVE CASPASE 3 Kit utilizes a
rabbit affinity purified polyclonal antibody raised against
amino acid 163-175 of murine caspase 3 (9). This neo epitope
is present on the p18 subunit of cleaved caspase 3 (9).
Cells undergoing apoptosis are fixed and permeablized prior
to the addition of the antibody. A secondary FITC labeled
goat anti rabbit antibody is used to visualize the bound
rabbit anti caspase 3 polyclonal.
|
| References |
-
Slee, E. A., C. Adrain, and S. J. Martin. 1999. Serial
Killers: ordering caspase activation events in
apoptosis. Cell Death and Differ. 6:1067-1074.
-
Walker, N. P., R. V. Talanian, K. D. Brady, L. C. Dang,
N. J. Bump, C. R. Ferenz, S. Franklin, T. Ghayur, M. C.
Hackett and L. D. Hammill. 1994. Crystal Structure of
the Cysteine Protease Interleukin-1ß-Converting Enzyme:
A (p20/p10)2
Homodimer. Cell 78:343-352.
-
Wilson, K. P., J. F. Black, J. A. Thomson, E. E. Kim, J.
P. Griffith, M. A. Navia, M. A. Murcko, S. P. Chambers,
R. A. Aldape, S. A. Raybuck, and D. J. Livingston. 1994.
Structure and mechanism of interleukin-1 beta converting
enzyme. Nature 370: 270-275.
-
Rotonda, J., D. W. Nicholson, K. M. Fazil, M. Gallant,
Y. Gareau, M. Labelle, E. P. Peterson, D. M. Rasper, R.
Ruel, J. P. Vaillancourt, N. A. Thornberry and J. W.
Becker. 1996. The three-dimensional structure of apopain/CPP32,
a key mediator of apoptosis. Nature Struct. Biol. 3(7):
619-625.
-
Kumar, S. 1999. Mechanisms mediating caspase activation
in cell death. Cell Death and Differ. 6: 1060-1066.
-
Alnemri, E.S. et al (1996) Cell 87:171
-
Trends Biochem Sci 22,388 (1997)
-
Nature 376, 37 (1995).
-
Srinivasan A, et al Cell Death and Differentiation
(1998) 5: 1004-1016
|
| Kit contents |
- 1 vial of Rabbit anti cleaved caspase 3 affinity
purified polyclonal antibody
- 1 vial of FITC labeled Goat anti Rabbit affinity
purified polyclonal antibody
- 1 vial of 10X fixative.
|
| The following
kits are available: |
Product
|
Catalog No.
|
Size
(No. of Tests) |
Price (Eur €) |
| APO ACTIVE 3 Antibody
Specific Active Caspase 3 Detection Kit |
FAB200-1 |
25 |
€145 |
FAB200-2
|
100 |
€425 |
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